Experiment Examining the Progress of the GFP DNA Sequence 21013686

Ruoqi Ma


In this experiment, two recombinant plasmas containing the coding sequence (CDS) for the green fluorescent protein (GFP) have been cloned into the commercial cloning vector pJET1.2 that we use. We used three different molecular methods to determine which way the GFP DNA sequence was going. Extending a gene’s expression by means of amplification using polymerase chain reaction. It was discovered that bacteria streaking allowed us to see and compare the variation in fluorescence level expressed in a regular plasma vector and an expression vector. As a result of their widespread utility, restriction enzymes have established themselves as a staple in the toolkit of each self-respecting molecular scientist. Different restriction enzymes are more suited for certain jobs than others due to subtle distinctions in their recognition sites and cutting mechanisms.

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DOI: https://doi.org/10.22158/se.v8n4p113


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