Determination of Organic Acids in Orange Fruit by HPLC

Abstract

Objective: To establish a method for rapid determination of 6 organic acids by HPLC. Methods: Ultimate C18 chromatographic column (4.6×250mm, 5μm) was used, the mobile phase was phosphate buffer solution (pH2.0), the flow rate was 0.5mL/min, and the detection wavelength was 210nm. Results: An HPLC analysis method for tartaric acid, malic acid, ascorbic acid, lactic acid, acetic acid, and citric acid was established, and the relative standard deviation was 1.38%~4.43%, the detection limit was 0.012~2.291 mg/L, and the recovery rate of organic acids was 89.7%~105.8%. Conclusion: This method has fast analysis speed, high reproducibility, and sensitivity, and is suitable for the determination of organic acids in oranges.