Determination of the Content of Hairy Vetch Isoflavone Glucoside in Qianliekang Tablets

Jiarui Cao, Xinghai Zhang

Abstract


Objective: To determine the content of maohui isoflavone glycoside based on its composition in Qianliekang tablets.

Methods: High-Performance Liquid Chromatography (HPLC) was employed for the determination, with a detection wavelength set at 260 nm; column temperature maintained at 25°C; and mobile phase consisting of acetonitrile-0.1% formic acid solution (14:86). The flow rate was standardized to 1.0 ml/minute.

Results: A good linear relationship between sample content and peak area was observed within the range of 0.051 μg to 0.816 μg for maohui isoflavone glycoside, with a relative standard deviation (RSD) of repeatability reaching 1.47%. The average recovery rate was found to be 98.21%, with an RSD of 2.31%.

Conclusion: This method demonstrates excellent stability, high accuracy, precision, and reproducibility, making it suitable for quality control of Qianliekang tablets.


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DOI: https://doi.org/10.22158/asir.v8n4p166

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