The Effect of Polyvalent Staphylococcus Vaccine on the Gene Expression of IL-8, IFN-γ, IL-1R and Phagocytes in Mice Spleen

Abstract

Objectives: To study the effect of Polyvalent staphylococcus vaccine on cellular immune function in mice. Methods: Forty SD kunming species of mice were randomly divided into low dose group (500 million/ml bacteria), middle dose group (1 billion/ml bacteria), high dose group (2 billion/ml bacteria) and control group, Mice were immunized with different doses of Polyvalent staphylococcus vaccine. The expression of IL-8, IFN - γ and IL-1R genes in spleen tissues of mice in different doses of experimental group and control group were detected by RT-PCR, and the phagocytic effect of phagocytes in abdominal cavity of mice was detected by abdominal smear Results: Compared with the normal control group, the expression level of IL-8, IFN - γ, IL-1R gene and phagocytic function of spleen in the experimental group were significantly higher than those in the control group (P < 0.01), the effect in the middle dose group were the best. Conclusion: Polyvalent staphylococcus vaccine can significantly improve the expression level of IL-8, IFN - γ, IL-1R gene in mouse spleen, and enhance the phagocytic function of phagocytes, and its effect showed dose-dependent manner.