Effect of Zhishenyuanhu Anticoagulant Formula on Hypercoagulability in Rats with Qi Stagnation and Blood Stasis and Arterial and Venous Thrombosis in Rats

Abstract

Background: Tumor patients present with hypercoagulable blood and are at risk of venous thromboembolism (VTE). We explored the effect of Zhishenyuanhu anticoagulant formula（ZSYH）on hypercoagulability in rats with qi stagnation and blood stasis and thrombosis in rats.

Methods: Part I. Epinephrine (0.6 mg/kg, hypodermic injection, h.i.) was administered every day for 7 days. Six groups were gavaged with saline, saline, rivaroxaban, low-dose, medium-dose, and high-dose ZSYH every day. We collected rats’ daily body weight, food intake, clotting time (CT), coagulation and hemorheology results. Part II. Six groups of rats were given the same drugs as in part I for 7 days. Half of rats in each group were used to construct a common carotid artery thrombusis model, while the other half were used to construct an inferior vena cava thrombusis model.

Results: ZSYH caused decreases in FIB, shear rate 1, whole blood low shear relative index, and RE. The high dose of ZSYH caused decreases in wet and dry weight of arterial and venous thrombosis.

Conclusion: ZSYH is effective in treating hypercoagulability in rats with qi stagnation and blood stasis. The high dose of ZSYH prevent the development of arterial thrombosis and venous thrombosis.