Extraction of Seabuckthorn Seed Oil and Analysis of Its Fatty Acid Composition

Abstract

Seabuckthorn seed oil was extracted by fat extraction method. The effects of quality of seabuckthorn seed, breaking time, soaking time and extraction solvent on the extraction rate were investigated by single factor experiment and response surface methodology. The fatty acids in seabuckthorn seed oil were determined. The research results show that the most suitable extraction conditions for using a fat extraction instrument are: seabuckthorn seed mass is 1.40 g, crushing time is 11 s, soaking time is 26 min, extraction time is 4 h, temperature is 70?, and the extraction solvent is petroleum ether. The extraction rate can reach 6.56%; the extracted seabuckthorn seed oil mainly contains three main fatty acids: linoleic acid, linolenic acid and oleic acid, these three fatty acids are also the main sn-2 fatty acids.